Part:BBa_K2110814:Design

PETase site mutation M154L - R

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

First apply PCR for mutant section. Then PCR again with original primers of PETase to overlap for the entire mutant PETase.

Source

Artificially Designed